Kromasil Columns

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Kromasil is a fully back integrated manufacturer of totally spherical silica particles for high performance chromatography usage. Our products are available as bare silica but also with various surface modifications for normal phase, reversed phase, and chiral applications.

Low metal impurities

Kromasil uses a proprietary manufacturing process. The metal content in all reagents and raw materials is minimized due to a rigorous quality control procedure. The table shows information regarding the metal content in three typical batches.

Hydrophobicity

This high hydrophobicity has two major advantages:

1.High surface hydrophobicity provides good separating power. The retention of analytes can then be adjusted by the mobile phase conditions, upon need.

2.High surface hydrophobicity provides good long-lasting performance, i.e. high chemical stability

Endcapping

To minimize undesired interactions between residual silanols and analytes.

Symmetrical peaks when using Kromasil

Kromasil RP-phases show excellent peak shape for both acidic and basic compounds.

Chemical stability

The Kromasil Classic platform is based on perfectly spherical silica-based materials to improve efficiency and decrease costs in laboratory analysis and purification steps.

Kromasil 60A°

The Kromasil Classic 60 Å family of products is the choice for small, organic molecules when a large, accessible surface area is key for separating peaks in analysis. It also has the added properties of loadability and capacity required for purification.

Particle sizes

SIL: 5 µm, 7 µm, 10 µm, 13 µm and 16 µm

CN: 5 µm, 10 µm and 16 µm

Diol: 5 µm and10 µm

Specifications

Phase	USP	pH range	Carbon load (%)	Pore size (A°)	Surface area (m²/g)	Endcapped
Kromasil Silica	L3	1.5 – 10.0	N/A	60	540	No
Kromasil CN	L10	1.5 – 10.0	12	60	540	No
Kromasil Diol	L20	1.5 – 10.0	10	60	540	No
Kromasil HILIC D	L20	1.5 – 10.0	N/A	60	540	No

Exploit selectivity differences

Kromasil 100A°

The well-known Kromasil^® Classic 100 Å family of products is used to separate and purify molecules of up to about 10,000 Da. In fact, drug candidates for the pharmaceutical, natural products and API industries are separated and purified using Kromasil^® Classic 100 Å columns and bulk material.

Specifications

Phase	USP	pH range	Carbon load (%)	Pore size (A°)	Surface area (m²/g)	Endcapped
Kromasil Silica	L3	1.5 – 10.0	N/A	100	320	No
Kromasil C8	L7	1.5 – 10.0	12	100	320	Yes
Kromasil C18	L1	1.5 – 10.0	20	100	320	Yes
Kromasil Phenyl	L11	1.5 – 10.0	14	100	320	Yes
Kromasil C4	L26	1.5 – 10.0	8	100	320	Yes
Kromasil NH₂	L8	1.5 – 10.0	N/A	100	320	N/A

Kromasil in small particle sizes for UHPLC and HPLC

Kromasil 100 Å is available in a variety of standard particle sizes from 1.8 to 16 µm (larger particles are available upon request). Therefore, scientists can now employ the same quality products as their counterparts across the organization, making it easier, faster and more cost-effective for a drug to reach market.

Separation within 2 minutes

Same selectivity in a fraction of the time

Consistency from batch to batch

An important aspect in preparative chromatography is the stationary phase batch-to-batch consistency. A vast number of tests are performed in the quality assurance and control of Kromasil.

Kromasil 300 A°

Protein and biomolecule separations from analytical to process scale

The Kromasil Classic 300 Å family of products is designed to be the perfect choice for proteins and biomolecules larger than 8 – 10 kDa. This 300 Å material has a narrow pore size distribution that ensures good mass transfer for larger molecules, resulting in narrow peaks and no size-exclusion effects.

Specifications

Phase	USP	pH range	Carbon load (%)	Pore size (A°)	Surface area (m²/g)	Endcapped
Kromasil Silica	L3	1.5 – 10.0	N/A	300	110	No
Kromasil C8	L7	1.5 – 10.0	4.7	300	110	Yes
Kromasil C18	L1	1.5 – 10.0	8.7	300	110	Yes
Kromasil C4	L26	1.5 – 10.0	2.9	300	110	Yes
Kromasil diC4	L26	1.5 – 10.0	2.9	300	110	Yes

Kromasil ClassicShell

Specifications

Phase	USP	pH range	Particle size (µm)	Carbon load (%)	Pore size (A°)	Surface area (m²/g)	Endcapped
Kromasil Classic C18	L1	1.5 – 10.0	2.5	7	90	150	Yes

Kromasil ClassicShell is a family of columns based on solid-core particles intended for fast analytical separations to support effective and efficient laboratory turnaround.

High-efficiency analytical columns

They are packed with 2.5 µm solid-core particles and, similarly to the fully porous Kromasil Classic 1.8 and 2.5 µm particle size columns, Kromasil ClassicShell products can offer high analytical efficiency but at a lower back-pressure cost, and can therefore be used on any HPLC instrument.

Discovery and development

Pharmaceutical drug discovery and development, food quality control and environmental monitoring require efficient sample analysis. Kromasil ClassicShell columns offer researchers and analysts the possibility of fast runs with complete resolution, as the results illustrated here.

Product Characteristics

For regular silica-based stationary phases, exposure to extreme pH (especially basic) will have a negative impact on the chemical stability and therefore column lifetime. However, the silica/organosilane surface of the Kromasil Eternity platform offers a chemical stability that will secure a long-lasting stationary phase, even under tough pH conditions and higher temperatures.

The silica matrix

The Eternity platform is based on the Kromasil 100 Å silica matrix, well known for high mechanical stability, and a well-defined pore structure.

The organosilane interfacial gradient

An organosilane is immobilized on the silica, and, under certain proprietary conditions merged into an organic/inorganic interfacial gradient. This process step has been finetuned to give Kromasil EternityXT its extreme chemical stability, extending the pH range and packing lifetime.

The Finished Product

Finally the product is functionalized with various surface chemistries (C18 in illustration), followed by a proprietary endcapping process.

Excellent performance even at high pH values

With the wide pH window, the Eternity platform gives users more flexibility to optimize selectivity and loading capacity compared to regular silica materials.

Choose your selectivity with pH

Improved resolution at high pH

The low pH chromatogram shows a non-favorable situation, with co-elution of two pairs of peaks. However, at high pH, well separated peaks can easily be obtained.

Kromasil EternityShell

Designed for users who expect to perform separations between pH 1 and 12 and look for fast separations using solid-core technology, enabling small particle efficiency on instruments with lower back-pressure specifications.

The EternityShell particle combines solid-core and Eternity organo-silane reinforcement technologies

Fast separations under harsh conditions

Specifications

Phase	USP	pH range	Carbon load (%)	Pore size (A°)	Surface area (m²/g)	Endcapped
Kromasil EternityXT C8	L7	1.0 – 12.0	13	100	320	Yes
Kromasil EternityXT C18	L1	1.0 – 12.0	18	100	320	Yes
Kromasil EternityXT PhenylHexyl	L11	2.0 – 12.0	12 - 15	100	320	Yes
Kromasil Eternity C18	L1	2.0 – 12.0	14	100	330	Yes
Kromasil Eternity PhenylHexyl	L11	2.0 – 12.0	12	100	330	Yes
Kromasil EternityShell C18	L1	1.0 – 12.0	8	90	150	Yes

Product Characteristics

High-performing chiral phases

Polysaccharide-based Kromasil^® AmyCoat and CelluCoat stretch the limits for chiral chromatography. The silica is based on a proprietary matrix and coated with a functionalized amylose or cellulose selector.

Kromasil^® AmyCoat and CelluCoat give high resolution, excellent selectivity and stable performance when switching between compatible mobile phases. Users do not have to worry about pressure limits, as both Kromasil^® AmyCoat and CelluCoat can withstand flow rates equivalent to pressures of up to 400 bar – i.e. the limit for most standard HPlC systems.

Fast and easy method development

To speed up and simplify method development, Nouryon has removed some of the restrictions for coated polysaccharide phases. In analytical scale chromatography, 3 μm particles and the absence of pressure limits allow fast chromatography with good separation results.

Selectivity and resolution comparison
– Kromasil^® AmyCoat 3 μm and Chiralpak AD-H (5 μm)

Fast analytical chromatography

By using smaller particles (for higher resolution), shorter columns and increasing flow rate as long as your system pressure or the selectivity allows it, tremendous improvements in analysis throughput can be achieved.

Stable performance

When it comes to stability, Kromasil^® AmyCoat and CelluCoat are compatible with normal, polar organic and reversed mobile phases. switching between compatible normal to polar organic mobile phases does not lead to any reduction in performance and there is no need for solvent dedicated columns.