ThermoFisher Scientific columns

Accucore UHPLC and HPLC columns

Thermo Scientific™ Accucore™ columns are based on solid core (superficially porous) particles yielding highly efficient, rugged separations for high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) applications. They offer lower backpressures than fully porous sub-2 µm particles. These are the columns of choice when sample volumes are limited, or when higher throughput is desired.

We offer the widest portfolio of solid core chemistries (1.5, 2.6, 4 µm), which benefits you with options to optimize your resolution and separations.

 The key components of Core Enhanced Technology

Particle Evolution

In the search for ever faster and better separations the size and shape of column packing materials has evolved in the decades since the invention of HPLC.

Why Core Enhanced Technology Works

Resistance to mass transfer is minimized by the solid core design of Core Enhanced Technology particles as the diffusional path of analytes is limited by the depth of the outer porous layer. The effect of this minimization is most noticeable for larger molecules.

Faster than 5 μm and 3 μm

Reducing analysis time and solvent costs results in higher throughput and lower cost per analysis.

Reducing analysis time and solvent costs results in higher throughput and lower cost per analysis.

Better sensitivity allows reliable detection and determination of small peaks, for example low level impurities.

 Accucore UHPLC and HPLC columns

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Hypersil GOLD HPLC columns

These general-purpose columns for HPLC and LC-MS applications provide exceptional peak shape affording confidence in your analytical data. With minimal peak tailing, these columns are packed to achieve exceptional efficiency, peak shape and resolution.

Thermo Scientific™ Hypersil GOLD™ columns offer a broad range of phases and particle sizes (1.9, 3, 5 µm). They are your go-to column for starting method development. 

Phase properties

Hypersil GOLD columns offer chromatographers flexibility in choosing the correct column, whether they are using conventional or ultra-high pressure liquid chromatography (LC) systems.

Phase overview

Hypersil GOLD columns are designed to provide exceptional peak shape with minimal peak tailing. These columns are packed to achieve exceptional efficiency, peak shape and resolution for your high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) applications. Hypersil GOLD columns are a great starting point for high efficiency separations and your go-to column for starting method development.

Hypersil GOLD 

Enhanced resolution

Obtaining narrow peak widths is especially challenging for basic pharmaceutical compounds. The reduced silanol activity on Hypersil GOLD columns reduces tailing for basic analytes, thus improving resolution.

pH stability

Hypersil GOLD columns are well suited to extended pH applications and have been shown to produce robust assays at high pH. At low pH, excellent column stability and reproducibility are illustrated over 1500 injections at pH 1.8.

High pH stability assay (pH 10.6) of Hypersil GOLD columns

Hypersil GOLD aQ

"Enhanced retention and resolution of polar analytes"

• Analysis of water soluble vitamins and organic acids
• Use with highly aqueous mobile phase

Retention and resolution of polar analytes

Because Hypersil GOLD aQ columns are packed with a polar endcapped C18 phase, they offer superior retention of polar compounds. Dispersive interactions are the primary mechanism of retention with alkyl chain bonded phases. The polar functional group used to endcap Hypersil GOLD aQ media provides an additional controlled interaction mechanism by which polar compounds can be retained and resolved. The resulting optimized peak shape provides excellent resolution sensitivity and efficiency, making Hypersil GOLD aQ columns ideal for the quantitative analysis of trace levels of polar analytes.

Polar endcapped C18 stationary phase for alternative selectivity

Ideal for highly aqueous mobile phases

The polar endcapping of Hypersil GOLD aQ media also makes it usable in 100% aqueous mobile phases without the risk of loss of performance or poor stability.

Excellent peak shapes

Hypersil GOLD aQ columns provide only controlled secondary interactions to ensure excellent peak shape for all analyte types, making them ideal for the quantitative analysis of trace levels of polar analytes.

Hypersil GOLD PFP

"Unique selectivity with perfluorinated columns "

• Analyzing difficult to resolve mixtures of halogenated compounds
• Non-halogenated polar aromatic compounds
• Analysis of complex taxane samples

Hypersil GOLD Amino

"Highly versatile aminopropyl stationary phase"

• Retains anions and organic acids in weak anion-exchange
• Excellent for carbohydrate analysis in HILIC

Outstanding peak shape and sensitivity

Based on the same highly pure silica backbone, Hypersil GOLD Amino columns offer improved peak shape over type A silica columns. For high speed, high efficiency separations, Hypersil GOLD Amino columns are available with 1.9 μm particle size

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Acclaim column families at a glance

Thermo Scientific Acclaim columns are designed for high-efficiency high performance liquid chromatography (HPLC) separations. Their unique functionalities set them apart for difficult and complex separations, often providing resolution of compounds not possible by other conventional HPLC columns.

Selecting an Acclaim column

What are the goals of the separation?

• Critical peaks requiring resolution
• Speed of analysis
• Solvent consumption/minimal waste
• Sensitivity requirements

Acclaim reversed-phase, HILIC and SEC columns

Reversed-phase chromatography

Monomeric bonded phases provide higher column efficiencies, than polymeric phases, but polymeric phases are very stable under pH extremes.

Carbon load and end-capping

Phases with higher carbon loads are more strongly hydrophobic, resulting in higher capacity, longer retention times, and often better resolution.

When the functional groups are attached to the silica particle, not all silanol groups on the surface of the silica particle are covered. Free silanol groups will interact with polar analytes, altering the retention times and often causing peak tailing for organic bases. To minimize these secondary interactions, the free silanol groups are end-capped. All Acclaim column packings are end-capped.

HILIC chromatography

HILIC is the recommended mode of separating polar compounds. These compounds are unretained under conventional reversed-phase conditions, but are retained using HILIC columns, without the requirement for ion-pair additives in the mobile phase. In this technique the stationary phase is polar and the aqueous portion of the mobile phase acts as the stronger solvent. Thus, the polar analytes can be retained and separated, even with 5-20% aqueous mobile phase.

Size exclusion chromatography (SEC)

Size exclusion chromatography (SEC) is a major mode of HPLC that employs porous particles in the column to separate molecules by virtue of their size in solution.

Acclaim reversed-phase and general purpose HPLC column

Acclaim reversed-phase and general purpose HPLC columns specifications

Acclaim SEC specifications

Acclaim Mixed-Mode HPLC columns

Mixed-Mode chromatography

• Excellent performance: selectivity, resolution and retention
• Good for separations of active pharmaceutical ingredients (APIs), mixtures, formulations, ions
• Good when retention requirements are contradictory for a single-mode column
• Offers flexibility in method development 

Bi-modal Mixed-Mode phases

The Acclaim family contains three bi-modal Mixed-Mode columns and two tri-modal columns. As shown here, bi-modal columns have both a hydrophobic arm – providing reversed-phase retention, and an ion-exchange or diol group at the tip – providing ion-exchange or HILIC retention.

Tri-modal Mixed-Mode phases Nanopolymer Silica Hybrid technology: Tri-modal Mixed-Mode phases

Acclaim Trinity P1 and P2 columns are based on Thermo Scientific™ Nanopolymer Silica Hybrid (NSH™) technology. These are high-purity porous spherical silica particles coated with charged nanopolymer particles. The inner pores have a bimodal functionality and the outer nanopolymers have a differing ion-exchange functionality. The spatial separation of the two ion-exchange regions allows both retention mechanisms to function simultaneously and be controlled independently.

Acclaim Mixed-Mode HPLC columns

Acclaim Mixed-Mode HPLC column specifications

Acclaim Mixed-Mode WAX-1

Designed for separating anionic molecules with powerful adjustable selectivity control

• Adjustable selectivity
• Selectivity orthogonal to reversed-phase (RP) columns
• Ideal selectivity for anionic molecules
• Excellent column efficiency and peak asymmetry
• Multimode retention mechanisms: reversed-phase, weak anion exchange, and HILIC modes

Acclaim Mixed-Mode WCX-1

Designed for separating cationic molecules with adjustable selectivity control

• Adjustable selectivity
• Ideal selectivity for separating basic molecules
• Selectivity complementary to C18 RP columns
• Multimode separation mechanism: reversed-phase, weak cation exchange, anion-exclusion and HILIC

Acclaim application-specific HPLC columns

Innovative chemistries tailored for challenging and critically important applications

Application-specific columns utilize novel and unique chemistries to provide superior resolution with ease of use for key pharmaceutical, environmental and food/beverage applications. These columns are designed and tested for specific application.

Specifications for Acclaim application-specific HPLC columns

Acclaim Organic Acid

Optimized and application-tested for the analysis of hydrophilic organic acids

Acclaim Acid column is the recommended column for determining small hydrophilic organic acids, C1 to C7 aliphatic acids, and hydrophilic aromatic acid and is also valuable for the analysis and quality assurance of food and beverage products, pharmaceutical preparations, plating baths, and manufacturing chemicals, chemical intermediates, and environmental samples.

• Tested to guarantee consistent hydrophilic organic acid separations
• Compatible with 100% aqueous mobile phases
• Hydrolytic stability at low-pH conditions
• Ideal selectivity for separating a wide spectrum of organic acids
• Excellent column efficiency and peak shapes for organic acids

Acclaim AmG C18

Designed to provide rugged and reproducible reversed-phase chromatography of aminoglycoside antibiotics

Aminoglycoside antibiotics are commonly used as clinical and veterinary medicines to treat bacterial infections. HPLC using ion-pairing reversed-phase separations is an effective technique for simultaneous qualitative and quantitative determination of aminoglycosides.

• Rugged and reproducible
• Excellent selectivity for the HPLC of aminoglycosides
• Superior resistance to acidic conditions for long column lifetime
• Easy-to-use with only aqueous mobile phase; TFA only, or TFA/HFBA or when PFPA is needed
• Compatible with simple rugged methods; no solvents are required
• High-efficiency and throughput 

Acclaim Carbamate columns

Designed for baseline separation of carbamate pesticides specified in US EPA method 531.2

• Baseline separation of carbamate pesticides specified in U.S. EPA Method 531.2
• Use with either LC/post-column derivatization/fluorescence or LC-MS detection
• Available in 2.2, 3 and 5 μm particle size
• Compatible with both binary (methanol/water) and ternary (acetonitrile/methanol/water) mobile phase gradients
• High-efficiency, extremely low column bleed, and rugged column packing

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