Column Protection

Guard columns

Drop-in guard cartridges and holders offer convenience, economy, and effective protection for extending analytical column lifetimes. The 10 mm design offers maximum protection with minimal increase in retention. Hypersil GOLD drop-in guard cartridges are provided in packs of 4 each.

UHPLC filter

Replaceable 0.2 μm Thermo ScientificTM UHPLC filter cartridges can be used to protect Hypersil GOLD 1.9 μm columns against particulate contamination, extending column lifetime. It’s low dead volume design maintains chromatographic performance without degrading peak shape and causes minimal efficiency loss through dispersion. The UHPLC filter adds minimal increase in backpressure and so can be fitted to any length column.

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Column Protection

• Guard and filters to protect your analytical column
• Economical extension of column life time

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Guard columns are short (10 mm length) chromatographic columns that can be connected in front of the HPLC column. The guard column purpose is to extend the lifetime of the HPLC column by serving as a filter and a pressure dampener.

The guard column contains a cartridge which is packed with the same stationary phase as the columns. It will accumulate particle impurities from the inlet liquid phase at the head of the cartridge. These particles can be coming from poor quality solvents, sample precipitates, or salts generated by buffer incompatibility with the mobile phase’s organic component. Samples containing heavy oils or greases can also affect the lifetime of the column as these highly hydrophobic excipients will bind irreversibly to the stationary phase. A guard column will minimize these effects.

When the system pressure starts rising, it could be a sign that the guard column has reached its lifetime. The guard cartridge needs to be replaced to prolong the chromatographic performance of the main column.

Kromasil guard columns are built of 3 parts:

• A cartridge: This is the expendable column packed with the same packing as the HPLC column the user intends to protect. It's 1 cm long. Cartridges are sold in 5 and 3 pack for analytical and semi-preparative columns, respectively.
• A holder: A stainless steel container for the cartridge.
• A coupler: A PEEK connector or a short capillary tubing (for larger prep columns) to attach the guard column at the inlet of the HPLC column.

A Guard starter kit for analytical columns contains one holder, one coupler and a 5 pack of cartridges of the actual silica packing.

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Pre-column cartridge head for direct connection onto the column:

- For 5-mm long cartridges with ID of 2.0, 3.0, 3.9, 4.0 and 4.6 mm, item no.: 15020508K05

- For 10- and 20-mm long cartridges with ID of 2.0, 3.0, 3.9, 4.0 and 4.6 mm, item no.: 15020508

Pre-column cartridge holder (stand-alone):

- For 10-mm long cartridges with ID of 2.0, 3.0, 3.9, 4.0 and 4.6 mm, item no.: 1546.4100K1

- For 20-mm long cartridges with ID of 2.0, 3.0, 3.9, 4.0 and 4.6 mm, item no.: 1546.4100K2 The respective pre-columns can be found in the VDS optilab price list for HPLC columns

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