Post Column Derivatization

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Pickering Laboratories Reagents & Chemicals are specially prepared to meet the demanding requirements of high-sensitivity pre- and post-column HPLC derivatization methods.

TRIONE® Ninhydrin Reagent

TRIONE® Ninhydrin reagent is specially formulated for amino acid analysis. It contains Ninhydrin, Hydrindantin (reduced Ninhydrin), a Lithium Acetate buffer, and Sulfolane, a water-miscible organic solvent.

Two Preparations are available to suit your usage and storage requirements: 

T100 AND T100C

  • Pour into your reservoir and use; the ultimate in convenience with a minimum of handling
  • 4-month* shelf life

T200

  • Combine two solutions, swirl, and use
  • 12-month* shelf life before mixing preparation; one month in the reservoir

o-phthalaldehyde(OPA)

Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions. Pickering’s OPA is specially prepared to meet the demanding requirements of high-sensitivity pre- and post-column HPLC derivatization methods. Our repurification process actually begins with commercially available 99 % material.

o-Phthalaldehyde (opa ) Diluent

Three application specific diluents are available : CB910 and GA104 for Carbamate and Glyphosate analyses respectively, and OD104 for analysis of amino acids and other amines.

 

Naphthalene-2,3-Dicarboxaldehyde

This reagent is especially effective for post-column or precolumn derivatization of proteins, peptides and other large amines since the derivative is stable and doesn’t internally quench. Naphthalene-2,3-dicarboxaldehyde can also be used as a reagent for chemiluminescence analysis.

Vanillin

Reagent formulation with Vanillin, Methanol and Sulfuric Acid allows specific post-column analysis of polyether antibiotics, such as Monensin, Narasin and Salinomycin.

p-Dimethylaminobenzaldehyde (DMAB)

For Post-column Analysis of Sulfa Drugs in feed and animal tissues. p-Dimethylaminobenzaldehyde (DMAB; Ehrlich’s Reagent) reacts rapidly with many primary amines to form a complex with maximum absorbance at 450 nm.

Thiofluor™ 2-(Dimethylamino) ethanethiol HCl 

For Automated Post-Column Derivatization of Primary Amines. Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions.

SERAPREP™ & URIPREP™

Reagents for the Preparation of Native Samples for Amino Acid Analysis. SERAPREP™ and URIPREP™ replace commonly used protein precipitation reagents such as Acetonitrile, Perchloric acid, and Picric acid, and eliminate the need for dialysis, ultrafiltration, and the repeated centrifugation steps, followed by pH adjustment.

SERAPREP™ is used for preparing serum and other samples with high buffering capacity, e.g. sardine oil. URIPREP™ is used for urine and other samples with low buffering capacity, such as fruit juices, beer and wines.

Hydrolysis Reagent CB130

Hydrolysis Reagent contains C47™ which prevents the precipitation of insoluble minerals (e.g. Ca2+, Mg2+) from samples of hard drinking water and ground water. The C47™ additive complexes these minerals and keeps them in solution. The new Hydrolysis Reagent CB130.2 is formulated according to USEPA Method 531.2 and contains 0.075 M Sodium Hydroxide. This reagent is applied during the first stage of post-column derivatization for the analysis of Carbamate pesticides.

Hypochlorite Diluent GA 116

The Preparation of the Oxidizing Reagent for Post-Column Glyphosate Herbicide Analysis. This diluent is used to prepare the oxidizing reagent required to convert Glyphosate to a primary amine prior to reacting it with OPA.

ChlorAC™ Buffer

For Preservation of Aqueous Carbamate Samples. Trace analysis of Carbamate insecticide residues in drinking water is a mandated requirement in the U.S. Since several of the common Carbamates—Carbaryl, Oxamyl, Hydroxycarbofuran—are labile in water due to oxidation or hydrolysis, the samples and standards must be preserved in order to obtain valid results.

Restore™

For Removal of Metal Ion Contamination and Restoration of Proper Ion Balance in Glyphosate Analytical Columns and Guards

Li220 & Na220L

Use Li220 for prepared physiological samples and to dilute amino acid calibration standards for use with Lithium columns and buffers. Use Na220L for hydrolysate samples and to dilute amino acid calibration standards for use with Sodium columns and buffers.

Read More for additional and ordering informations

**Please contact technical sales or This email address is being protected from spambots. You need JavaScript enabled to view it. for order or additional information.

Fortune Scientific Co., Ltd.

7/25-26 Phaholyothin Rd. Anusawari,
Bang Khen, Bangkok 10220

Tel: 02-9861250-4
Fax: 02-986-1255
E-mail: sales@fortunesci.com

 

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