Solid phase extraction

Analyte Properties

Polar, uncharged species – can also retain some ionic/charged species

Sample Preparation/Application (Polar, aqueous) 

Add internal standard to the sample if quantification is desired. Optimize sample application by removing particulates if necessary (centrifugation or filtration) and/or diluting viscous matrices with water or buffer to ensure proper pH for desired interactions. This will require some experimentation.

Column Conditioning 

2-5 column volumes of a strong eluting solvent followed by 2-5 column volumes water.

Column Wash

Use water or a weak eluting solvent which will wash most interferences from the sorbent without loss of analytes. Wash pH may greatly affect cleanup and/or recovery

Elution

Use a strong eluting solvent to elute the analytes of interest. Addition of 0.1% TFA to the solvent will increase its elution strength for polar analytes.

Suggested Elution Solvents ; 

• MeOH
• THF*
• Ethyl Acetate*
• Dichloromethane*
• Chloroform*

*Elution strength will depend on the nature of the analytes.

Application

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Analyte Properties

Moderately polar to non-polar, uncharged

Sample Preparation/Application (Polar, aqueous)

Add internal standard to the sample if quantification is desired. Optimize sample application by removing particulates if necessary (centrifugation or filtration) and/or diluting viscous matrices with water or buffer to ensure proper pH for optimum retention.

Column Conditioning

2-5 column volumes of a strong solvent such as methanol at low vacuum (-3 inches Hg). Apply deionized or distilled water to remove excess solvent. Momentary high vacuum (5 to 8 inches Hg) may be necessary to restart flow. If the sorbent is accidentally dried, then resolvate and reflush column.

Column Wash

Use a solvent which will wash most interferences from the sorbent without loss of analyte. Wash pH may greatly affect cleanup and/or recovery.

Elution

Use a strong eluting solvent which will elute the analytes of interest.

Suggested Elution Solvents ; 

• MeOH
• Acetonitrile
• THF*
• Ethyl Acetate*
• Dichloromethane*

Application

Ordering informations

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Analyte Properties

Moderately polar to polar compounds

Sample Preparation/Application (Non-polar or Moderately Polar Organic Solvent)

Add internal standard to the sample if quantification is desired. Optimize sample application by removing particulates if necessary (centrifugation or filtration).

Column Conditioning

2-5 column volumes non-polar solvent such as heptane at low vacuum (-3 inches Hg). Release the vacuum or begin flushing immediately upon completion. The more air that passes through the column before sample loading, the less solvated the sorbent will be.

Column Wash

Use a solvent which will wash most interferences from the sorbent without loss of analyte.

Elution

Use a more polar solvent (e.g., introduction of more ethyl acetate, acetone or MeOH). 

Suggested Elution Solvents;

• Heptane
• Toluene
• Dichloromethane
• Acetone
• Ethyl Acetate
• MeOH

Applications

Ordering informations

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Analyte Properties

Moderately polar to non-polar and ionized and charged compounds

Sample Preparation/Application

Add internal standard to the sample if quantification is desired. Optimize sample application by removing particulates if necessary (centrifugation or filtration) and/or diluting viscous matrices with water or buffer to ensure proper pH for optimum retention. Neutral species are unaffected by pH. Charged species may show maximum retention under acidic or basic conditions and method development is required to establish this.

Column Conditioning

2-5 column volumes polar solvent such as methanol at low vacuum (-3 inches Hg). Release the vacuum or begin flushing immediately upon completion. The more air that passes through the column before sample loading, the less solvated the sorbent will be. Apply deionized or distilled water to remove excess solvent. Momentary high vacuum (5 to 8 inches Hg) may be necessary to restart flow. If the sorbent is accidentally dried, then resolvate and reflush the column.

Column Wash

Use water to disrupt the hydrophilic interactions and methanol to disrupt residual hydrophobic and hydrophilic interactions.

Elution

Use an organic solvent, possibly with some water to elute hydrophobically bound analytes. Then use an aqueous buffer with a pH that would neutralize ionically bound analytes or an aqueous with high ionic strength or a solvent with a counter ion that would bind to a sorbent.

Applications

Ordering informations

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Thermo Scientific™ SOLA and SOLAμ cartridges and plates provide clean, highly reproducible sample extracts with lower elution volumes due to the unique and innovative frit-less SPE design.

SOLA and SOLAμ SPE products provide unparalleled performance characteristics compared to conventional SPE, phospholipid removal and protein precipitation products. This includes:

• Higher levels of reproducibility
• Lower elution volumes
• High extract cleanliness

Improved Reproducibility and Recovery

The information presented shows lower variability sample to sample, and higher recovery levels for SOLA SPE products compared to conventional loose packed competitor SPE products, ensuring you achieve the correct result time after time.

Improved reproducibility

In comparison SOLA SPE products provide signi cantly higher levels of reproducibility which is vitally important for high throughput studies.

Higher sensitivity and lower solvent consumption

SOLA SPE products achieve excellent recovery levels even with low volumes of extract solvents, resulting in a more concentrated analyte and increased sensitivity.

Phase modifications

Ordering informations

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